Identification of peptides specific for antibodies in vitiligo using a phage library
Identifieur interne : 002F85 ( Main/Exploration ); précédent : 002F84; suivant : 002F86Identification of peptides specific for antibodies in vitiligo using a phage library
Auteurs : Z. Jadali [Iran] ; M. B. Eslami [Iran] ; M. H. Sanati [Iran] ; P. Mansouri ; M. Mahmoudi [Iran] ; . N. Maghsoudi [Iran] ; F. Esfahanian [Iran]Source :
- Clinical and Experimental Dermatology [ 0307-6938 ] ; 2005-11.
Abstract
Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with monoclonal and polyclonal antibodies. We used this technique to identify the epitopes that react specifically with the vitiligo autoantibodies. By screening the random peptide phage library and using ELISA, two clones that showed a higher frequency of reactivity with the antibodies in the sera of patients with vitiligo were identified. The peptides do not show any similarity with the autoantigens so far implicated in vitiligo, indicating that these epitopes may mimic conformational epitopes in proteins.
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DOI: 10.1111/j.1365-2230.2005.01903.x
Affiliations:
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Jadali</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Departments of Immunology and Biostatics & Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Eslami, M B" sort="Eslami, M B" uniqKey="Eslami M" first="M. B." last="Eslami">M. B. Eslami</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Departments of Immunology and Biostatics & Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Sanati, M H" sort="Sanati, M H" uniqKey="Sanati M" first="M. H." last="Sanati">M. H. Sanati</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>National Research Center for Genetic Engineering and Biotechnology, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Mansouri, P" sort="Mansouri, P" uniqKey="Mansouri P" first="P." last="Mansouri">P. Mansouri</name><affiliation><wicri:noCountry code="no comma">Departments of Dermatology</wicri:noCountry></affiliation></author><author><name sortKey="Mahmoudi, M" sort="Mahmoudi, M" uniqKey="Mahmoudi M" first="M." last="Mahmoudi">M. Mahmoudi</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Departments of Immunology and Biostatics & Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Maghsoudi, N" sort="Maghsoudi, N" uniqKey="Maghsoudi " first=". N." last="Maghsoudi">. N. Maghsoudi</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Biotechnology Group, Malek‐Ashtar University of Technology, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Esfahanian, F" sort="Esfahanian, F" uniqKey="Esfahanian F" first="F." last="Esfahanian">F. Esfahanian</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Endocrinology, Imam Khomeini General Hospital, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Clinical and Experimental Dermatology</title><title level="j" type="alt">CLINICAL EXPERIMENTAL DERMATOLOGY</title><idno type="ISSN">0307-6938</idno><idno type="eISSN">1365-2230</idno><imprint><biblScope unit="vol">30</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="694">694</biblScope><biblScope unit="page" to="701">701</biblScope><biblScope unit="page-count">8</biblScope><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2005-11">2005-11</date></imprint><idno type="ISSN">0307-6938</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0307-6938</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Patients with vitiligo produce specific autoantibodies that can be detected in their sera. These antibodies are believed to play a role in the pathogenesis of this disease. A random peptide library displayed on phage is a technique that can be used to identify the epitopes that react with monoclonal and polyclonal antibodies. We used this technique to identify the epitopes that react specifically with the vitiligo autoantibodies. By screening the random peptide phage library and using ELISA, two clones that showed a higher frequency of reactivity with the antibodies in the sera of patients with vitiligo were identified. The peptides do not show any similarity with the autoantigens so far implicated in vitiligo, indicating that these epitopes may mimic conformational epitopes in proteins.</div></front></TEI><affiliations><list><country><li>Iran</li></country></list><tree><noCountry><name sortKey="Mansouri, P" sort="Mansouri, P" uniqKey="Mansouri P" first="P." last="Mansouri">P. Mansouri</name></noCountry><country name="Iran"><noRegion><name sortKey="Jadali, Z" sort="Jadali, Z" uniqKey="Jadali Z" first="Z." last="Jadali">Z. Jadali</name></noRegion><name sortKey="Esfahanian, F" sort="Esfahanian, F" uniqKey="Esfahanian F" first="F." last="Esfahanian">F. Esfahanian</name><name sortKey="Eslami, M B" sort="Eslami, M B" uniqKey="Eslami M" first="M. B." last="Eslami">M. B. Eslami</name><name sortKey="Maghsoudi, N" sort="Maghsoudi, N" uniqKey="Maghsoudi " first=". N." last="Maghsoudi">. N. Maghsoudi</name><name sortKey="Mahmoudi, M" sort="Mahmoudi, M" uniqKey="Mahmoudi M" first="M." last="Mahmoudi">M. Mahmoudi</name><name sortKey="Sanati, M H" sort="Sanati, M H" uniqKey="Sanati M" first="M. H." last="Sanati">M. H. Sanati</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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